hCeO2@ Cu5.4O nanoparticle alleviates inflammatory responses by regulating the CTSB–NLRP3 signaling pathway

• DOI: 10.3389/fimmu.2024.1344098
• Publication Date: 2024-04-22T04:21:27Z
• Authors: Ying Li; Xiaomin Xia; Zhaojun Niu; Ke Wang; Jie Liu; Xue Li
• Keywords: Cerium oxide; Nanomaterials

Inflammatory responses, especially chronic inflammation, are closely associated with many systemic diseases. There ways to treat and alleviate but how solve this problem at the molecular level has always been a hot topic in research. The use of nanoparticles (NPs) as anti-inflammatory agents is potential treatment method. We synthesized new hollow cerium oxide nanomaterials ( h CeO 2 NPs) doped different concentrations Cu 5.4 O NPs [the molar ratio Cu/(Ce + Cu) was 50%, 67%, 83%, respectively], characterized their surface morphology physicochemical properties, screened safe concentration @Cu using CCK8 Macrophages were cultured, P.g -lipopolysaccharide-stimulated used model inflammation co-cultured NPs. then observe effect transcription levels CTSB, NLRP3, caspase-1, ASC, IL-18, IL-1β by PCR detect its on expression CTSB protein Western blot. IL-18 cell supernatant measured enzyme-linked immunosorbent assay. Our results indicated that could reduce production reactive oxygen species inhibit NLRP3 damage caused inflammatory response cells. More importantly, showed stronger effects NP doping increased. Therefore, development novel nanomaterial provides possible approach for