The distribution and maturation of tertiary lymphoid structures can predict clinical outcomes of patients with gastric adenocarcinoma

• DOI: 10.3389/fimmu.2024.1396808
• Publication Date: 2024-07-29T04:38:50Z
• Authors: Hui Sun; Yuxi Liu; Wanjing Cheng; Rong Xiong; Wenchao Gu; Xiaoyan Zhang; Xin Wang; Xu Wang; Cong Tan; Weiwei Weng; Meng Zhang; Shujuan Ni; Dan Huang; Midie Xu; Weiqi Sheng; Lei Wang
• Keywords: Male; 0301 basic medicine; Immunology; Dendritic Cells; RC581-607; Adenocarcinoma; Middle Aged; Prognosis; Germinal Center; 03 medical and health sciences; Tertiary Lymphoid Structures; Lymphocytes, Tumor-Infiltrating; Stomach Neoplasms; stomach adenocarcinoma; DC-LAMP+ dendritic cells; Tumor Microenvironment; Humans; Female; prognosis; Immunologic diseases. Allergy; tertiary lymphoid structure (TLS); maturation of TLSs; distribution of TLSs; Aged

Introduction Tertiary lymphoid structures (TLSs) are analogues of secondary organs that contain various immune cells. The spatial distribution, maturation and composition TLSs have differential effects on prognosis, the roles in gastric adenocarcinoma (GA) not been revealed. Methods Thus, we evaluated prognostic value GA through analysis bulk RNA sequencing(RNA-seq) data from public databases validated our findings tumour samples Fudan University Shanghai Cancer Center (FUSCC) cohort. distribution,maturation, were analysed by reviewing H&E-stained sections multiplex immunofluorescence (mIF) staining. Results We found TLSs, especially with germinal centres (GCs) located invasive margin (IM), correlated prolonged overall survival (OS). Second, RNA-seq showed high dendritic cell (DC) scores a favourable factor patients for both GCs. In FUSCC cohort, DC-LAMP+ DCs weresignificantly enriched IM-TLSs GCs, suggesting potential correlation between activation milieu DC abundance. Third, compared to without proportion FOXP3+CD8+ Treg cells was significantly decreased percentage PD1+CD20+ B increased Discussion Our results demonstrate arrangement affect prognosis indicate could be new additional histopathological evaluation.