Epigenetic factors and inflammaging: FOXO3A as a potential biomarker of sarcopenia and upregulation of DNMT3A and SIRT3 in older adults

• DOI: 10.3389/fimmu.2025.1467308
• Publication Date: 2025-02-17T06:47:31Z
• Authors: Diana Bogucka; Anna Wajda; Barbara Stypińska; Marcin Jerzy Radk...; Tomasz Targowski; Ewa Modzelewska; Tomasz Kmiołek; Adam Ejma-Multański; Gabriela Filipowicz; Yana Kaliberda; Ewa Dudek; Agnieszka Paradow...
• Keywords: sarcopenia; geriatrics; inflammation; ageing; frailty; FOXO3A; Immunologic diseases. Allergy; RC581-607

Background Epigenetic factors influence inflammaging and geriatric disorders such as sarcopenia frailty. It is necessary to develop a biomarker/panel of biomarkers for fast easy diagnostics. Currently, hard-to-access equipment required diagnose sarcopenia. The development will prevent many older adults from being excluded the diagnostic process. Methods In this study, we analyzed selected gene expression profiles, namely, SIRT1 , SIRT3 SIRT6 DNMT3A FOXO1 FOXO3A ELAVL1 in whole blood. study included 168 subjects divided into five groups: patients hospitalized at Geriatrics Clinic Polyclinic with sarcopenia, frailty syndrome, or without those (geriatric control), non-hospitalized healthy controls (HC) aged 25 30 years over 50 years. Results We revealed lower mRNA level (p<0.001) sarcopenic compared controls. Furthermore, detected upregulation (p=0.003) (p=0.015) HC old Interestingly, observed 2 cluster formations during correlation analysis ( ). also noted correlations clinical parameters levels group, vitamin D (r=0.64, p=0.010), creatine kinase (r=–0.58, p=0.032) (r=–0.59, p=0.026), creatinine (r=0.57, erythrocyte sedimentation rate (ESR) (r=0.69, p=0.004), lactate dehydrogenase (LDH) (r=–0.86, p=0.007). syndrome appendicular skeletal muscle mass (ASMM) (r=0.59, p=0.026) level. controls, serum iron (r=–0.79, p=0.036). Conclusions Our potential biomarker high epigenetic ) adults.