Quorum Sensing as a Target for Controlling Surface Associated Motility and Biofilm Formation in Acinetobacter baumannii ATCC® 17978TM

• DOI: 10.3389/fmicb.2020.565548
• Publication Date: 2020-09-30T06:56:14Z
• Authors: Celia Mayer; Andrea Muras; Ana Parga; Manuel Romero; Soraya Rumbo-Feal; Margarita Poza; José Ramos-Vivas; Ana Otero
• Keywords: Acinetobacter baumannii; Quorum Quenching

The important nosocomial pathogen Acinetobacter baumannii presents a quorum sensing (QS) system (abaI/abaR) mediated by acyl-homoserine-lactones (AHLs) and several quenching (QQ) enzymes. However, the role of this complex network in control expression virulence-related phenotypes such as surface-associated motility biofilm formation are not clear. Therefore, effect mutation AHL synthase AbaI, exogenous addition QQ enzyme Aii20J on A. ATCC® 17978TM was studied detail. multidrug-resistant clinical isolates differing their pattern also tested. We provide evidence that functional QS is required for robust 17978TM. Important differences were found with well-studied strain nosocomialis M2 regarding relevance depending environmental conditions vitro biofilm-formation capacity strains highly variable related to antibiotic resistance or profiles. A high variability sensitivity action enzyme, revealing virulence regulation between confirming studies restricted single representative development novel antimicrobial strategies. Extracellular DNA emerges key component extracellular matrix biofilms since combined DNase reduced all tested strains. Results demonstrate strategies combination other enzymatic treatments could represent an alternative approach prevention colonization survival surfaces treatment infections caused pathogen.