Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

• DOI: 10.3389/fmicb.2021.664810
• Publication Date: 2021-06-21T09:55:40Z
• Authors: Yuting Shang; Qifan Sun; Hanfang Chen; Qingping Wu; Moutong Chen; Shuanghong Yang; Mingzhu Du; Fei Zha; Qinghua Ye; Jumei Zhang
• Keywords: Lytic cycle; Podoviridae; Isolation

Salmonella is a widely distributed foodborne pathogen. The use of phages as biocontrol agents has recently gained significant interest. Because the genus high diversity, efforts are necessary to identify lytic focusing on different serovars. Here, five were isolated from soil samples, and vB_SalP_TR2 was selected novel phage with potential against host serovar Albany, well other tested serovars, including Corvallis, Newport, Kottbus, Istanbul. Morphological analyses demonstrated that belongs Podoviridae family, an icosahedral head (62 ± 0.5 nm in diameter 60 1 length) short tail (35 length). latent period burst size 15 min 211 PFU/cell, respectively. It contained linear dsDNA 71,453 bp, G + C content 40.64%. Among 96 putative open reading frames detected, only 35 gene products found database searches, no virulence or antibiotic resistance genes being identified. As biological control agent, exhibited temperature pH tolerance. In vitro , it lysed most S. Albany after 24 h at 37°C multiplicities infection 0.0001, 0.001, 0.01, 0.1, 1, 10, 100. food matrices (milk chicken meat), treatment also reduced number compared controls. These findings highlighted antibacterial agent for samples.