In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii

• DOI: 10.3389/fmicb.2021.752070
• Publication Date: 2021-10-07T22:49:19Z
• Authors: Kelly Conde-Pérez; Juan C. Vázquez-Ucha; Laura Álvarez-Fraga; Lucía Ageitos; Soraya Rumbo-Feal; Marta Martínez-Gu...; Noelia Trigo-Tasende; Jaime Rodríguez; Germán Bou; Carlos Jiménez; Alejandro Beceiro; Margarita Poza
• Keywords: Acinetobacter baumannii

Acinetobacter baumannii is a multidrug-resistant pathogen that represents serious threat to global health. A. possesses wide range of virulence factors contribute the bacterial pathogenicity. Among them, siderophore acinetobactin one most important, being essential for development infection. In this study we performed an in-depth analysis cluster in strain ATCC 17978. For purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical performed. Individual mutants lacking biosynthetic genes entA, basG , basC basD basB showed significant loss virulence, due disruption production. Similarly, gene bauA coding receptor, was also found be crucial pathogenesis. addition, Δ basJ/ fbsB double demonstrated high level genetic redundancy between siderophores where role specific can fulfilled by their fimsbactin redundant genes. Overall, highlights entA pathogenicity provides potential therapeutic targets design new antivirulence agents against microorganism.