The Involvement of Mycobacterium Type III-A CRISPR-Cas System in Oxidative Stress

• DOI: 10.3389/fmicb.2021.774492
• Publication Date: 2021-12-09T13:03:24Z
• Authors: Fan Yang; Lingqing Xu; Lujie Liang; Wanfei Liang; Jiachen Li; Daixi Lin; Min Dai; Dianrong Zhou; Yaxin Li; Yong Chen; Hui Zhao; Guo-bao Tian; Siyuan Feng
• Keywords: 0303 health sciences; 03 medical and health sciences; envelope integrity; immunoregulation; type III-A CRISPR-Cas; oxidative stress; Microbiology; QR1-502; Mycobacterium; 3. Good health

Type I and type II CRISPR-Cas systems are employed to evade host immunity by targeting interference of bacteria's own genes. Although Mycobacterium tuberculosis (M. tuberculosis), the causative agent tuberculosis, possesses integrated III-A system, its role in mycobacteria remains obscure. Here, we observed that seven cas genes (csm2∼5, cas10, cas6) were upregulated bovis BCG under oxidative stress treatment, indicating system stress. To explore functional TCC (Type including cas6, csm2-6) mutant was generated. Deletion results increased sensitivity response hydrogen peroxide reduced cell envelope integrity. Analysis RNA-seq dataset revealed impacted on oxidation-reduction process composition wall which is essential for mycobacterial envelop Moreover, disrupting led poor intracellular survival vivo vitro. Finally, showed first time contributed regulation regulatory T population, supporting a modulating immunity. Our finding reveals important homeostasis. work also highlights as an factor immunoregulation mycobacteria, thus may be potential target therapy.