Regulatory Connections Between the Cyanobacterial Factor PipX and the Ribosome Assembly GTPase EngA

• DOI: 10.3389/fmicb.2021.781760
• Publication Date: 2021-12-09T13:02:36Z
• Authors: Carmen Jerez; Paloma Salinas; Antonio Llop; Raquel Cantos; Javier Espinosa; Jose I. Labella; Asunción Contreras
• Keywords: 0303 health sciences; PII; Protein interaction; phenotypic analysis; Synechococcus elongatus; confocal microscopy; Genética; Microbiology; QR1-502; Confocal microscopy; 03 medical and health sciences; Phenotypic analysis; Nitrogen regulation network; protein interaction; bacterial two hybrid; Bacterial two hybrid

Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to important environmental challenges, like those imposed by the succession of days and nights. Not surprisingly, certain regulatory proteins are found exclusively in this phylum. One these unique proteins, PipX, provides a mechanistic link between signals carbon/nitrogen energy, transduced signaling protein PII, control gene expression global nitrogen regulator NtcA. required for cell survival unless PipX is inactivated or downregulated, functions protein-protein interactions with transcriptional regulators, transporters, enzymes. also interactions, previous studies suggested existence additional interacting partners included it into relatively robust six-node synteny network apparently unrelated regulation system. To investigate while providing proof concept recently developed cyanobacterial linkage network, here we analyzed physical an intriguing component essential ribosome assembly GTPase EngA. The results provide insights EngA showing that interacts GD1 domain guanosine diphosphate-dependent manner interferes Synechococcus elongatus at low temperature, environmentally relevant context. Therefore, work expands interaction establishes possible connection translation machinery. We discuss model integrating information on PII-PipX presented work.