Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice

• DOI: 10.3389/fmicb.2021.784211
• Publication Date: 2021-11-16T07:46:07Z
• Authors: Fan Wan; Ruqing Zhong; Mengyu Wang; Yexun Zhou; Yuxia Chen; Bao Yi; Fujiang Hou; Lei Liu; Yong Zhao; Liang Chen; Hongfu Zhang
• Keywords: Akkermansia; GPX1; Malondialdehyde

Caffeic acid (CA) is one of the major phenolic acids coffee with multiple biological activities. Our previous study found that 500 mg/kg chlorogenic (CGA) had potential capacity alleviating colonic inflammation. Moreover, CGA can be degraded into caffeic by gut microbiota in colon. Therefore, we hypothesize CA exert protective effects on To test hypothesis, 251 was supplemented to DSS-induced colitis mice. The results showed treatment recovered disease activity index (DAI), colon length, and histopathology scores tissue. Additionally, significantly decreased pro-inflammatory cytokines malondialdehyde (MDA) levels increased level IL-10, total antioxidant (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) serum. qPCR indicated dramatically downregulated mRNA expression IL-1β, IL-6, TNF-α as well upregulated SOD1, GPX1, GPX2, CAT, IL-10. In addition, supplementation Nrf-2, HO-1, NQO1, which its anti-inflammatory capacities potentially activating Nrf-2/HO-1 pathway. prevented barrier damage enhancing Occludin gene expression. Furthermore, altered microbiome composition decreasing relative abundance Bacteroides Turicibacter, Alistipes Dubosiella. Meanwhile, increases Dubosiella Akkermansia. conclusion, could effectively alleviate improving defense against oxidative stress inflammatory response.