Multi-Omics Analysis Reveals the Dynamic Changes of RNA N6-Methyladenosine in Pear (Pyrus bretschneideri) Defense Responses to Erwinia amylovora Pathogen Infection

• DOI: 10.3389/fmicb.2021.803512
• Publication Date: 2022-02-10T10:07:22Z
• Authors: Chenyang Han; Feng Zhang; Xin Qiao; Yancun Zhao; Qinhai Qiao; Xiaosan Huang; Shaoling Zhang
• Keywords: Fire blight; N6-Methyladenosine

N6-methylated adenine (m6A) is the most prevalent modification of mRNA methylation and can regulate many biological processes in plants, such as processing, development, stress response. Some studies have increased our understanding its various roles model plants recent years. Nevertheless, distribution m6A impact on regulation plant defense responses against pathogen inoculation are virtually unknown pear. In this study, MeRIP-seq RNA-seq data from healthy inoculated were analyzed to assess changes transcript levels posttranscriptional pear response fire blight Erwinia amylovora. Following analysis 97,261 peaks, we found that preferred modify duplicate genes rather than singleton m6A-methylated underwent stronger purifying selection. A total 2,935 specific sites detected at transcriptome level after inoculation, which may increase defense-related abundance enhance resistance. addition, 1,850 transcripts only mock-inoculated groups. The hypomethylated mainly related transcriptional processes, chloroplast organization sucrose biosynthetic processes. extent was significantly positively correlated with level, suggesting a regulatory role for