Effects of challenge with Clostridium perfringens, Eimeria and both on ileal microbiota of yellow feather broilers

• DOI: 10.3389/fmicb.2022.1063578
• Publication Date: 2022-12-01T06:31:50Z
• Authors: Xin Feng; Tonghao Li; Hui Zhu; Lidan Liu; Shengqun Bi; Xiaolin Chen; Huihua Zhang
• Keywords: Clostridium perfringens; Eimeria maxima; Poultry farming

In the poultry industry worldwide, Clostridium perfringens has been causing major economic loss as it can cause necrotic enteritis (NE). The coccidial infection considered most important predisposing factor of NE caused by C. perfringens. this study, we aimed to advance our knowledge on ileal microbiota yellow feather broilers under and/or Eimeria challenge. Total 80 healthy day old were randomly assigned four groups including: Control, challenge group (C. Per), (Cocc), and plus (Comb). On 14, Cocc Comb orally gavaged 1 ml PBS solution containing 25,000 oocysts brunetti maxima. Starting 17, Per 10 mL per bird (4 × 107 CFU/mL, ATCC® 13124™ Strain) every for 6 days. 16S rRNA gene sequencing was performed extracted DNA digesta samples. results showed that alone did not affect alpha diversity microbiome in but co-infection with significantly decreased microbiota. also relative abundance beneficial bacteria including Bacteroidetes at phylum level Faecalibacterium genus level. At species level, Candidatus Arthromitus challenged groups. This microbial shift information Perfringens provide reference data development therapeutic approaches yellow-feather broiler chickens.