Antibody and T Cell Immune Responses to SARS-CoV-2 Peptides in COVID-19 Convalescent Patients

• DOI: 10.3389/fmicb.2022.842232
• Publication Date: 2022-04-18T05:29:52Z
• Authors: Ekaterina Garanina; Shaimaa Hamza; Robert J. Stott-M...; Ekaterina Martynova; Maria Markelova; Yuriy Davidyuk; Venera Shakirova; Neha Kaushal; Manoj Baranwal; Ilsiyar M. Khaert...; Albert Rizvanov; Toshana L. Foster; Svetlana Khaiboul...
• Keywords: ELISPOT; Coronavirus

Identifying immunogenic targets of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is critical to advance diagnostic and disease control strategies. We analyzed humoral (ELISA) T-cell (ELISpot) immune responses spike (S) nucleocapsid (N) SARS-CoV-2 proteins as well human endemic coronavirus (eCoV) peptides in serum from convalescent 2019 (COVID-19) patients Tatarstan, Russia. identified multiple that were reactive with antibodies T cells COVID-19. In addition, age gender associated differences the reactivity S N protein identified. Moreover, several tested negatively correlated severity lung damage. Cross-reactivity eCoV was found be lower COVID-19 compared controls. this study, we demonstrate changing pattern peptide based on age, previous exposure eCoVs. These data highlight how cytotoxic cell some these could contribute pathogenesis.