Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy

• DOI: 10.3389/fmicb.2022.846167
• Publication Date: 2022-03-03T06:58:54Z
• Authors: Fernanda Pimentel...; Mattia Pirolo; Monica Monaco; Maria Del Grosso; Simone Ambretti; Donatella Lombardo; Tiziana Cassetti; Raffaele Gargiulo; Eleonora Riccobono; Paolo Visca; Annalisa Pantosti
• Keywords: Pathogenicity island; Lineage (genetic); Multilocus sequence typing; Molecular Epidemiology

Staphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore clonal complex (CC) distribution and pattern virulence determinants S. isolates from OM in Italy. Whole-genome sequencing performed on 83 cases six hospitals. Antibiotic susceptibility tests showed that 30.1% were methicillin-resistant (MRSA). frequent CCs detected CC22, CC5, CC8, CC30, CC15, which represent lineages circulating Italian MRSA limited number (CC22, CC1). Phylogenetic analysis followed sequence type-CC groupings revealed a non-uniform different No significant difference mean genes carried by or MSSA observed. Some genes, namely cna, fib, fnbA, coa, lukD, lukE, sak, tst, correlated with CC. However, categories factors, such as adhesins, exoenzymes, toxins, frequently unevenly distributed among all lineages. Indeed, each lineage variable combination likely reflecting functional redundancy, arguing for importance those traits pathogenicity OM. In conclusion, no specific genetic trait could explain their high prevalence isolates. Our findings highlight follow epidemiology infections country. It conceivable any CC can cause variety infections, including