Evaluation of Potential Probiotic Properties of a Strain of Lactobacillus plantarum for Shrimp Farming: From Beneficial Functions to Safety Assessment

• DOI: 10.3389/fmicb.2022.854131
• Publication Date: 2022-03-29T10:27:55Z
• Authors: Cong Wei; Kai Luo; Mingyang Wang; Yongmei Li; Miaojun Pan; Yumeng Xie; Guangcai Qin; Yijun Liu; Li Li; Qingbing Liu; Xiangli Tian
• Keywords: Pseudoalteromonas; Kanamycin

In recent years the safety of probiotics has received increasing attention due to possible transfer and spread virulence factors (VFs) antibiotic resistance genes (ARGs) among microorganisms. The a strain Lactobacillus plantarum named W2 was evaluated in phenotype genotype present study. Its probiotic properties were also both vivo vitro, including adherence properties, antibacterial beneficial effects on growth immunity Pacific white shrimp, Penaeus vannamei. Hemolysis tests, tests whole genome sequence analysis showed that had no significant did not carry high factors. found be sensitive chloramphenicol, clindamycin, gentamicin, kanamycin tetracycline, resistant ampicillin erythromycin. Most ARGs have risk few but enrichment human-associated environments. autoaggregation 82.6% hydrophobicity 81.0%. Coaggregation rate with Vibrio parahaemolyticus (24.9%) significantly higher than Vibrio's (17.8%). This suggested adhesion potential mucosal/intestinal surfaces able attenuate V. parahaemolyticus. addition, several adhesion-related protein genes, 1 S-layer protein, collagen-binding 9 mucus-binding proteins identified genome. efficiently antagonistic activity against 7 aquatic pathogenic strains. Antagonistic components indicated active substances might organic acids. can promote shrimp when supplemented × 1010 cfu/kg live cells. Levels serological immune indicators expression levels 12 hepatopancreatic immune-related up-regulated, mortality exposed reduced. Based above, L. applied safely as enhance performance, capacity disease P.