Mycolicibacterium lacusdiani sp. nov., an Attached Bacterium of Microcystis aeruginosa

• DOI: 10.3389/fmicb.2022.861291
• Publication Date: 2022-05-12T13:55:34Z
• Authors: Yao Xiao; Jian Chen; Min Chen; Shao-Ji Deng; Zhi-Qian Xiong; Bao-Yu Tian; Bing-Huo Zhang
• Keywords: Microcystis aeruginosa; Microcystis

In eutrophic water, attached bacteria of Microcystis play an important role in the formation, development, and degradation blooms. A novel actinobacterium, designated as JXJ CY 35 T , was isolated from culture mass aeruginosa FACHB-905 (Maf) collected Lake Dianchi, Yunnan Province, China. Strain gram-positive, acid-fast staining, aerobic, with short rod-shaped cells, positive for catalase, negative oxidase. The isolate able to grow at 10.0–36.0°C, pH 4.0–10.0, tolerate up 5.0% (w/v) NaCl, optimal growth 28°C, 7.0–8.0, 0% NaCl. Cell-wall peptidoglycan contains aspartic acid, glutamic glycine, alanine, mannose, ribose, galactose, arabinose whole-cell sugars. Polar lipids consist diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), glycolipid (GL1-3), phosphoglycolipid (PGL), phosphatidylinositol (PI), unidentified lipid (L1). predominant menaquinone MK-9. Major fatty acids (>10%) were C 17:1 ω7c (37.0%) 18:1 ω9c (18.9%). complete genome sequence strain 6,138,096 bp size a DNA G + content 68.3%. Based on 16S rRNA gene sequences, it has 98.2% similarity Mycolicibacterium arabiense JCM 18538 . digital DNA-DNA hybridization (dDDH) average nucleotide identity (ANI) values between closest five type strains M. goodii ATCC 700504 mageritense DSM 44476 austroafricanum 44191 Mycobacterium neglectum CECT 8778 52.1, 20.3, 20.6, 19.8%, 92.7, 75.5, 75.6, 76.0, 75.2%, respectively. On basis above taxonomic data differences physiological characteristics closely related strain, determined represent species genus which name lacusdiani sp. nov., is proposed. (=KCTC 49379 = CGMCC 1.17501 ). Different inoculation dosages could exhibit different effects Maf its toxin synthesis release. promote by providing available phosphorus, nitrogen, probably vitamins, plant hormones.