Epidemiology of blaCTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010–2017

• DOI: 10.3389/fmicb.2022.865254
• Publication Date: 2022-06-17T07:05:03Z
• Authors: Qi Jiang; Bi-xia Ke; De-shu Wu; Dong Wang; Liang-xing Fang; Ruan-yang Sun; Min-ge Wang; Jing-er Lei; Zheng Shao; Xiao-ping Liao
• Keywords: Multilocus sequence typing; Salmonella enterica; Molecular Epidemiology

Salmonella enterica can lead to intestinal diarrhea, and the emergence spread of cephalosporin-resistant have brought great challenges clinical treatment. Therefore, this study investigated prevalence transmission bla CTX-M genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, 2010 2017. A total 221 -positive isolates were recovered 1,263 facal samples diarrhoea patients 45 general hospitals 11 cities. The most popular gene was CTX-M-55 (39.6%, 72/182) CTX-M-1 group, followed by CTX-M-14 (22.5%, 41/182) CTX-M-65 (19.2%, 35/182) CTX-M-9 group. that carried CTX-M-9G had significantly higher resistance rates multiple antibacterials compared with CTX-M-1G ( p < 0.01). Meanwhile, PFGE analysis not only showed clonal CTX-M-55/14/65 -positve outpatients’ origins different Guangdong province, but also characteristic isolates’ bacterial persistence. Multilocus sequence typing (MLST) indicated these possessed ST34 ST19. Furthermore, genomic Beast phylogenomic provided evidence a close relationship between pork. Most transmitted non-typeable or IncI1/IncFII/IncHI2 plasmids size ranging ~80 ~280 kb. Moreover, whole-genome sequencing (WGS) further revealed coexisted other 25 types ARGs, which ARGs highly prevalent detection >50%, it first reported TEM-141 Typhimurium. This underscores importance surveillance for microbes diarrhea patients.