Formulation of Chemically Defined Media and Growth Evaluation of Ligilactobacillus salivarius ZJ614 and Limosilactobacillus reuteri ZJ625

• DOI: 10.3389/fmicb.2022.865493
• Publication Date: 2022-05-06T07:41:02Z
• Authors: Iliya Dauda Kwoji; Moses Okpeku; Matthew Adekunle ...; Olayinka Ayobami ...
• Keywords: Lactobacillus reuteri; Growth curve (statistics); Bacterial growth

Lactic acid bacteria are increasingly becoming important dietary supplements due to their health benefits when consumed in adequate quantity. The increasing attention on these microbes has necessitated an in-depth understanding of physiological processes, such as nutritional requirements and growth patterns, better harness probiotic potentials. This study was carried out determine the for L. salivarius ZJ614 reuteri ZJ625 from a chemically defined medium evaluate kinetics by fitting different sigmoidal models. complete CDM contains 49 ingredients glucose, Tween 80®, mineral salts, buffers, amino acids, vitamins, nucleotides at concentrations. In addition, minimal isolates were determined series single-omission experiments (SOEs) compose MDM. Growth curve data generated culturing automated 96-well micro-plate reader 37°C 36 h, photometric readings (optical density: OD600) taken. summarized tables charts using Microsoft Excel, while evaluation open-source software (Curveball) Python. results revealed that omission groups resulted 2.0, 20.17, 60.24% (for ZJ614) 0.95, 42.7, 70.5% ZJ625) relative growths, respectively. Elimination individual components also indicates varying levels strains. LogisticLag2 Baranyi-Roberts models best fits ZJ614, All strains showed appreciable MDM observed de Man-Rogosa-Sharpe (MRS) broth. We described CDM, estimated parameters.