Bacillus subtilis PS-216 Spores Supplemented in Broiler Chicken Drinking Water Reduce Campylobacter jejuni Colonization and Increases Weight Gain

• DOI: 10.3389/fmicb.2022.910616
• Publication Date: 2022-07-08T06:43:13Z
• Authors: Katarina Šimunović; Orhan Sahin; Andi Erega; Polonca Štefanič; Qijing Zhang; Ines Mandic Mulec; Sonja Smole Možina; Anja Klančnik
• Keywords: Campylobacteriosis

Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source infection. To reduce risk associated with C. transmission via meat, effective interventions during production are needed, use probiotics promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. PS-216 strain demonstrated best activity against isolates when examined using vitro co-cultures. evaluate suitability probiotic use, its susceptibility to eight clinically important antimicrobials simulated gastric conditions was investigated. sensitive all tested antibiotics. Although vegetative cells conditions, spores highly resistant. We further evaluated spore preparation (2.5 × 106 CFU/mL water) prevent and/or colonization broiler chickens vivo. Compared untreated group, significantly lower counts detected caeca broilers continuously treated drinking water. Furthermore, showed improved weight gain, compared control group. Together, these results demonstrate potential improve gain.