Salidroside ameliorates memory impairment following long-term ethanol intake in rats by modulating the altered intestinal microbiota content and hippocampal gene expression

• DOI: 10.3389/fmicb.2023.1172936
• Publication Date: 2023-06-09T04:54:35Z
• Authors: Yu Jiao; Zhenglin Zhao; Xin Li; Lulu Li; Dan Xiao; Siyuan Wan; Tong Wu; Tong Li; Ping Li; Rongjie Zhao
• Keywords: metagenomics; gut microbiota; hippocampus; Salidroside; memory impairment; ethanol; Microbiology; QR1-502; 3. Good health

Salidroside (Sal), the main component of a famous herb Rhodiola rosea L, enhances memory performance and reduces fatigue. Therefore, this study assessed effect Sal on impairment induced by long-term intake ethanol (EtOH) in rats investigated its relevant mechanisms using gut microbiota metagenomic analysis hippocampal transcriptomic analysis.Eighteen male SD were divided into normal control group (CON group), EtOH model (Model treatment (Sal group). The Model groups intragastrically (i.g.) received 2 g/kg for 30 consecutive days, whereas CON was given an equal volume distilled water. Meanwhile, administered i.g. mg/kg 60 min after intake. All tested eight-arm maze their function every 3 days. On 30th day, analyses hippocampus performed.Compared with group, reduced total time to complete task, decreased number arm entries, abated working error that significant from 9th day. Additionally, intervention improved composition, such as increased abundance Actinobacteria Bifidobacterium, which related metabolism amino acids terpenoid carbohydrate, endocrine function, signal transduction neurotransmitters. In hippocampus, differentially expressed 68 genes (54 increased, 14 decreased), compared affected these changes: 15 11 decreased. And, enrichment revealed structural components ribosome, mRNA splicing process, protein translation, mitochondria immunological reaction. Finally, correlation found positively correlated abnormal upregulation Tomm7 but negatively Alistipes_indistinctus, Lactobacillus_taiwanensis, Lactobacillus_paragasseri, Lactobacillus johnsonii.Sal caused rats, may be regulation dysbiosis dysfunction.