Landscapes of gut microbiome and bile acid signatures and their interaction in HBV-associated acute-on-chronic liver failure

• DOI: 10.3389/fmicb.2023.1185993
• Publication Date: 2023-05-18T11:53:41Z
• Authors: Zhiwei Bao; Runan Wei; Xiaoping Zheng; Ting Zhang; Yunjiao Bi; Sijia Shen; Pengfei Zou; Junjie Zhang; Huadong Yan; Ming D. Li; Zhongli Yang; Hainv Gao
• Keywords: Gut microbiome

Submassive hepatic necrosis (SMHN, defined as of 15-90% the entire liver on explant) is a likely characteristic pathological feature ACLF in patients with hepatitis B cirrhosis. We aimed to comprehensively explore microbiome and bile acids patterns across enterhepatic circulation build well-performing machine learning models predict SMHN status.Based presence or absence SMHN, 17 HBV-related end-stage disease who received transplantation were eligible for inclusion. Serum, portal venous blood, stool samples collected comparing differences BA spectra gut their interactions. adopted random forest algorithm recursive elimination (RF-RFE) status.By total spectrum between (-) (+) patients, significant changes detected only fecal (P = 0.015). Compared group, group showed that UDCA, 7-KLCA, 3-DHCA, 7-KDCA, ISOLCA α-MCA feces, r-MCA, 7-KLCA 7-KDCA serum, γ-MCA vein enriched, TUDCA feces was depleted. PCoA analysis significantly distinct overall microbial composition two groups 0.026). Co-abundance bacterial species formed strong broad relationships BAs. Among them, Parabacteroides distasonis had highest node degree. further identified combinatorial marker panel high AUC 0.92.Our study demonstrated interactions intestinal BAs during enterohepatic SMHN. In addition, we non-invasive biomarkers distinguish status AUC.