The Helicobacter pylori single nucleotide polymorphisms SNPs associated with multiple therapy resistance in Colombia

• DOI: 10.3389/fmicb.2023.1198325
• Publication Date: 2023-07-07T16:34:02Z
• Authors: Kevin Guzman; Lidia Montenegro; Alvaro Pazos
• Keywords: 23S ribosomal RNA

The eradication of Helicobacter pylori (H. pylori) using multiple therapies is used as a prevention strategy. However, its efficacy has been compromised by the emergence single nucleotide polymorphisms in genes associated with H. pylori's resistance to antibiotics. To estimate antibiotic rates mutations high-cancer-risk population Colombia, we included 166 whole genome sequences from cohort individuals high risk gastric cancer. By reference strain ATCC 26695, identified specific evaluate for different antibiotics: 23S rRNA clarithromycin, 16S tetracycline, pbp1A amoxicillin, gyrA levofloxacin, and rdxA metronidazole. phylogenomic analysis was conducted core consisting 1,594 pylori-ATCC 26695. Our findings revealed that rate clarithromycin 3.62%, primarily A2143G A2142G gene. For 7.23%, A926G, A926T, A928C observed Amoxicillin found 25.9% cases, gene, including T556S, T593, R649K, R656P, R656H. In N87K, N87I, D91G, D91N, D91Y were identified, resulting 12.04% levofloxacin. most common gene metronidazole stop codon, at D59N D59S, 99.3%. indicated this drug should be excluded therapy. tetracycline did not exceed established threshold Colombia. increased levofloxacin amoxicillin may partially explain therapeutic failures tree showed isolate belongs own lineage (hspColombia). These offer valuable insights enhance characterization treatment protocols (hspColombia) local level.