Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways

• DOI: 10.3389/fmicb.2023.1237438
• Publication Date: 2023-11-16T17:35:54Z
• Authors: Lijun Wan; Sheng Wang; Zhixun Xie; Hongyu Ren; Liji Xie; Sisi Luo; Meng Li; Zhiqin Xie; Qing Fan; Tingting Zeng; Yanfang Zhang; Minxiu Zhang; Jiaoling Huang; You Wei
• Keywords: Bursa of Fabricius

Interferon-alpha inducible protein 6 (IFI6) is an important interferon-stimulated gene. To date, research on IFI6 has mainly focused human malignant tumors, virus-related diseases and autoimmune diseases. Previous studies have shown that plays role in antiviral, antiapoptotic tumor-promoting cellular functions, but few the structure or function of avian IFI6. Avian reovirus (ARV) virus can exert immunosuppressive effects poultry. Preliminary expression upregulated various tissues organs specific-pathogen-free chickens infected with ARV, suggesting ARV infection. analyze IFI6, particularly infection, chicken gene was cloned, a bioinformatics analysis conducted, roles replication innate immune response were investigated after overexpression knockdown vitro. The results indicated molecular weight approximately 11 kDa its similar to IFI27L1 protein. A phylogenetic tree amino acid sequence revealed evolution mammals birds clearly divided into two branches. evolutionary history homology are those other birds. localized cytoplasm abundantly expressed lung, intestine, pancreas, liver, spleen, glandular stomach, thymus, bursa Fabricius trachea. Further demonstrated DF-1 cells inhibited inhibition promoted replication. After modulated immunity-related factors. Notably, patterns MAVS similar, IRF1 IFN-β opposite this study further advance provide theoretical basis for infection immunity.