Multi-approach methods to predict cryptic carbapenem resistance mechanisms in Klebsiella pneumoniae detected in Central Italy

• DOI: 10.3389/fmicb.2023.1242693
• Publication Date: 2023-08-28T12:50:04Z
• Authors: Alessandra Cornac...; Anna Janowicz; Gabriella Centoro...; Maria Antonietta ...; Sofia Chiatamone ...; Massimo Ancora; Paola Ripà; Cesare Cammà; Francesco Pomilio; Alexandra Chiaverini
• Keywords: Genome-wide Association Study; Carbapenem; Klebsiella

The rapid emergence of carbapenem-resistant Klebsiella pneumoniae ( Kp ) strains in diverse environmental niches, even outside the clinical setting, poses a challenge for detection and real-time monitoring novel antimicrobial resistance trends using molecular whole genome sequencing-based methods. aim our study was to understand cryptic determinants responsible phenotypic carbapenem observed circulating Italy by combined approach involving sequencing (WGS) genome-wide association (GWAS). In this study, we collected 303 from inside settings between 2018–2022 Abruzzo region Italy. profile all isolates assessed both bioinformatic We identified 11 resistant carbapenems, which did not carry any known genetic explaining their phenotype. GWAS results showed that incongruent phenotype associated specifically with two capsular types, KL13 KL116 including genes involved capsule synthesis, encoding proteins assembly biosynthesis apparatus, capsule-specific sugar processing export, polysaccharide pyruvyl transferase, lipopolysaccharide protein. These preliminary confirmed potential identifying variants present could be traits . implementation advanced methods, such as increased surveillance will potentially improve infection treatment patient outcomes.