Prevalence of multidrug-resistant hypervirulent Klebsiella pneumoniae without defined hypervirulent biomarkers in Anhui, China: a new dimension of hypervirulence

• DOI: 10.3389/fmicb.2023.1247091
• Publication Date: 2023-10-05T06:41:17Z
• Authors: Md Roushan Ali; Yu Yang; Yuanyuan Dai; Huaiwei Lu; Zhien He; Yujie Li; Baolin Sun
• Keywords: Aerobactin

Klebsiella pneumoniae is an opportunistic pathogen that mainly causes nosocomial infections and hospital-associated pneumonia in elderly immunocompromised people. However, multidrug-resistant hypervirulent K. (MDR-hvKp) has emerged recently as a serious threat to global health can infect both healthy individuals. It scientifically established plasmid-mediated regulator of mucoid phenotype genes (rmpA rmpA2) other virulence factors (aerobactin salmochelin) are responsible for this phenotype. In study, we collected 23 MDR-hvKp isolates performed molecular typing, whole genome sequencing, comparative genomic analysis, phenotypic experiments, including the Galleria mellonella infection model, reveal its genetic features. Meanwhile, discovered two (22122315 22091569) showed wide range hypervirulence hypermucoviscosity without rmpA rmpA2 any factors. isolate 22122315 highest (infection model) with significant mucoviscosity, conversely, 22091569 exhibited mucoviscosity (string test) higher compared control. These carried carbapenemase (blaKPC - 2), β-lactamase (blaOXA 1, blaTEM 1B), extended-spectrum (ESBL) (blaCTX M 15, blaSHV 106), outer membrane protein-coding (ompA), fimbriae encoding (ecpABCDER), enterobactin coding (entAB, fepC). addition, single nucleotide polymorphism analysis indicated isolates, 22091569, were found have novel mutations loci FEBNDAKP_03184 (c. 2084A > C, p. Asn695Thr), EOFMAFIB_02276 1930C A, Pro644Thr), respectively. Finally, NCBI blast suggested these located wzc capsule polysaccharide (cps) region putative tyrosine kinase. This study would be strong reference enhancing current understanding identifying lacked regulators