Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis

• DOI: 10.3389/fmicb.2023.1320202
• Publication Date: 2024-01-08T06:12:48Z
• Authors: Shixiong Zhang; Mengnan Li; Liping Chang; Xinjing Mao; Yuning Jiang; Xiaogang Shen; Kunxu Niu; Xuan Lu; Runtao Zhang; Yahui Song; Kun Ma; Hongrong Li; Cong Wei; Yunlong Hou; Yiling Wu
• Keywords: Pyroptosis; Gut–brain axis; Capsule; Barrier function

Purpose The senescence-accelerated prone mouse 8 (SAMP8) is a widely used model for accelerating aging, especially in central aging. Mounting evidence indicates that the microbiota-gut-brain axis may be involved pathogenesis and progression of aging-related diseases. This study aims to investigate whether Bazi Bushen capsule (BZBS) attenuates deterioration intestinal function aging animal model. Methods In our study, SAMP8 mice were randomly divided into group, BZ-low group (0.5 g/kg/d BZBS), BZ-high (1 BZBS) RAPA (2 mg/kg/d rapamycin). Age-matched SAMR1 as control group. Next, cognitive was detected through Nissl staining two-photon microscopy. gut microbiota composition fecal samples analyzed by 16S rRNA gene sequencing. Ileum tissue morphology observed hematoxylin eosin staining, barrier immunofluorescence. expression senescence-associated secretory phenotype (SASP) factors, including P53, TNF-α, NF-κB, IL-4, IL-6, IL-10 measured real-time quantitative PCR. Macrophage infiltration proliferation differentiation cells assessed immunohistochemistry. We also inflammasome pyroptosis levels ileum western blotting. Results BZBS improved neuronal density mice. restored villus structure function, which damaged reduced SASP factors macrophages tissues, indicating lower level inflammation. enhanced cells, are essential maintaining homeostasis. modulated composition, inhibited activation inflammasomes intestine. Conclusion could restore dysbiosis prevent inhibiting NLRP3 inflammasome-mediated pyroptosis. These results suggested attenuated mice, at least partially, targeting axis.