Changes in soil organic carbon components and microbial community following spent mushroom substrate application

• DOI: 10.3389/fmicb.2024.1351921
• Publication Date: 2024-05-17T04:32:46Z
• Authors: Guiting Yang; Yan Ma; Xiaochi Ma; Xuanqing Wang; Chao Lu; Wenyi Xu; Jia Luo; Dejie Guo
• Keywords: Soil carbon; Carbon fibers

While spent mushroom substrate (SMS) has shown promise in increasing soil organic carbon (SOC) and improving quality, research on the interplay between SOC components microbial community following application of diverse SMS types remains scant. A laboratory incubation experiment was conducted with two SMSs from cultivation Pleurotus eryngii (PE) Agaricus bisporus (AB), each at three rates (3, 5.5, 8%). Advanced techniques, including solid-state 13C nuclear magnetic resonance (NMR) high-throughput sequencing, were employed to investigate fractions chemical structure, composition functionality. Compared SMS-AB, SMS-PE increased relative abundances carbohydrate O-alkyl C SOC. In addition, abundance bacterial phylum Proteobacteria those fungal phyla Basidiomycota Ascomycota. The cellulose-degrading (e.g., Flavisolibacter Agromyces) genera Myceliophthora, Thermomyces, Conocybe) as well. SMS-AB aromaticity index SOC, aromatic C, contents humic acid heavy fraction carbon. significantly Firmicutes Actinobacteria. Notably, Actinomadura, Ilumatobacter, Bacillus, which positively correlated acid, experienced an increase abundance. Functional prediction revealed that elevated metabolism reduced prevalence pathogens, particularly Fusarium. high-rate (8%) enhanced amino plant pathogenic fungi. Our provides strategies for utilizing enrich fortify health, facilitating achievement sustainable management.