Hyalangiumruber sp. nov, characterization of a novel myxobacterium strain s54d21 and their secondary metabolites

• DOI: 10.3389/fmicb.2024.1369499
• Publication Date: 2024-03-08T17:39:54Z
• Authors: Yi Zang; Xianjiao Zhang; Zhe Wang; Qingyi Tong; Yang Zhou; Qing Yao; Honghui Zhu
• Keywords: Myxobacteria; Strain (injury)

Myxobacteria are special bacteria with wide adaptability, which rich sources of structurally diverse natural products intriguing biological properties. Here, a gram-negative myxobacterium strain s54d21 T was isolated from the sediment wetland park in China using Escherichia coli baiting method. Based on 16S rRNA gene sequence and genomic data, demonstrated to be novel species rare genus Hyalangium , designated ruber sp. nov (type = GDMCC 1.1945 JCM 39263 ). The subsequent chemical investigation led isolation three 3,5,6-trisubstituted 2(1 H )-pyrazinones, namely, hyalanones A–C ( 1 – 3 ), together known macrolactin A 4 Those new structures their absolute configurations were unambiguously assigned by extensive analyses spectroscopic data density functional theory (DFT) calculations. In assays, compound exhibited moderate cytotoxic activities against human cell lines RKO, A549, NCM460 IC 50 values ranging 27.21 32.14 μ M.