The single-cell transcriptional landscape of lung cells from PCV2d-infected mice

• DOI: 10.3389/fmicb.2025.1554961
• Publication Date: 2025-03-24T08:31:20Z
• Authors: Yunlong Chen; Gang Fan; Bin Yang; Xinyi Fan; Haiyan Chen; Zhuoyuan Ma; Jiao Lou; Jingmei Xu; Yan Wang; Shiqiang Zhang

Introduction Porcine Circovirus (PCV2) infection is prevalent in pig farming and causes significant economic losses. In recent years, the PCV2d subtype has become most genotype worldwide, exhibiting higher virulence, leading to more severe viremia organ damage. Therefore, studying biological characteristics of great significance. Methods We established a model using BALB/c mice employed single-cell RNA sequencing (scRNA-seq) systematically analyze transcriptome 10 cell types lung tissues infected mice. developed comprehensive marker gene catalog for these types. Results Compared uninfected mice, induced extensive viral replication immunosuppressive responses Monocyte macrophages with high levels replication, pro-inflammatory cytokines, various population interactions occurring through CD40-CD40L CXCL14-CXCR4 were identified. These cells predominantly mediate antigen presentation processing pathways vivo , contributing PCV2d-driven inflammatory injury. Discussion Our data uncovered complex unique immune response scenario tissue after infection, deciphering potential mechanisms underlying Furthermore, this study provides rich database molecular basis different types' infection.