ANLN Enhances Triple-Negative Breast Cancer Stemness Through TWIST1 and BMP2 and Promotes its Spheroid Growth

• DOI: 10.3389/fmolb.2021.700973
• Publication Date: 2021-07-01T13:50:29Z
• Authors: Alishba Maryam; Y. Rebecca Chin
• Keywords: Triple-negative breast cancer; Synthetic Lethality; FOXM1

ANLN is frequently upregulated in triple-negative breast cancer (TNBC) and its high expression tumors are significantly associated with poor survival recurrence, thereby it has been proposed to function as a prognostic marker for cancer. However, the specific molecular mechanisms by which promotes TNBC tumorigenesis remain elusive. Using multiomic profiling, we recently uncovered TNBC-specific gene driven super-enhancer. Here, Crispr/Cas9 editing, showed that knockout of inhibits spheroid growth TNBC. Interestingly, effect on cell proliferation 2D cultures minimal. depletion mammosphere formation clonogenicity potently, suggesting important regulating stem cells (CSCs). We screened panel cell-related genes several CSC regulated ANLN. further identify TWIST1 BMP2 essential mediate ANLN’s stemness but not growth. These findings may contribute search effective targeted therapies treat