Serum Proteomic Analysis by Tandem Mass Tag-Based Quantitative Proteomics in Pediatric Obstructive Sleep Apnea

• DOI: 10.3389/fmolb.2022.762336
• Publication Date: 2022-04-11T05:09:25Z
• Authors: Hanrong Cheng; Shoumei Jin; Simin Huang; Tianyong Hu; Miao Zhao; Dongcai Li; Benqing Wu
• Keywords: Pathophysiology; Tandem mass tag

Pediatric obstructive sleep apnea (OSA) is a frequent respiratory disorder with an estimated prevalence of 3-6% in the general population. However, underlying pathophysiology OSA remains unclear. Recently, proteomic analysis using high-resolution and high-throughput mass spectrometry has been widely used field medical sciences. In present study, tandem tag (TMT)-based was performed serum patients OSA. The revealed set differentially expressed proteins that may be associated were enriched pathways including phagosome glycan synthesis/degradation, immune response, hedgehog signaling pathway, indicating such functions are key targets Moreover, experimental validation studies four ANTXR1, COLEC10, NCAM1, VNN1 reduced from moderate severe OSA, while MAN1A1 CSPG4 protein levels elevated inversely correlated apnea-hypopnea index (AHI) recruited subjects, level positively AHI, no significant correlation detected between AHI. summary, study for first time identified different severities by TMT-based analysis. functional enrichment suggested several results indicated six VNN1, CGPG4, play important roles which requires further mechanistic investigation.