Identification of Novel Immune Cell-Relevant Therapeutic Targets and Validation of Roles of TK1 in BMSCs of Systemic Lupus Erythematosus

• DOI: 10.3389/fmolb.2022.848463
• Publication Date: 2022-04-11T05:06:00Z
• Authors: Fangru Chen; Jian Meng; Wenjie Yan; Mengjiao Wang; Yunfei Jiang; Jintao Gao
• Keywords: CXCL1; Senescence

Objective: Systemic lupus erythematosus (SLE) displays the characteristics of abnormal activity immune system, contributing to diverse clinical symptoms. Herein, this study was conducted for discovering novel cell-relevant therapeutic targets. Methods: The abundance cells estimated in PBMCs SLE and healthy controls from GSE50772 dataset with CIBERSORT approach. Immune co-expression modules were screened WGCNA relevant characteristic genes determined LASSO algorithm. Inflammatory chemokines measured serum twenty patients through ELISA. Bone marrow mesenchymal stem (BMSCs) isolated TK1 expression BMSCs RT-qPCR western blotting. TK1-overexpressed TK-1-silenced apoptosis cell cycle flow cytometry. Apoptosis-, cycle- senescence-relevant proteins tested Results: We three strongly linked cells. Five (CXCL1, CXCL2, CXCL8, CXCR1 TK1) ROC curves proved excellent diagnostic performance model. presented widespread up-regulations patients, demonstrating activation inflammatory response. remarkably elevated than controls. overexpression enhanced IL-1β expression, apoptosis, arrest, senescent phenotypes opposite results TK1-silenced BMSCs. Conclusion: Collectively, our findings demonstrate that silencing alleviates inflammation, growth arrest senescence SLE, which highlights as a promising target against SLE.