A biochemical, theoretical and immunohistochemical study comparing the therapeutic efficacy of curcumin and taurine on T-2 toxin induced hepatotoxicity in rats

• DOI: 10.3389/fmolb.2023.1172403
• Publication Date: 2023-05-04T04:20:54Z
• Authors: Maryam H. Al-Zahrani; Maha J. Balgoon; Nagwa M. El-Sawi; Fawzia A. Alshubaily; Ebtihaj J. Jambi; Sohair M. Khojah; Raghad S. Baljoon; Nuha A. Alkhattabi; Lina A. Baz; Asmaa A. Alharbi; Amira M. Ahmed; Ayat M. Abo elkhair; Mohamed Ismael; Sahar M. Gebril
• Keywords: TBARS

Introduction: Foodborne trichothecene T-2 Toxin, is a highly toxic metabolite produced by Fusarium species contaminating animal and human food, causing multiple organ failure health hazards. toxins induce hepatotoxicity via oxidative stress hepatocytes cytotoxicity genotoxicity. In this study, curcumin taurine were investigated compared as antioxidants against T-2-provoked hepatotoxicity. Methods: Wistar rats administrated toxin sublethal oral dose (0.1 mg/kg) for 2 months, followed (80 (50 3 weeks. Biochemical assessment of liver enzymes, lipid profiles, thiobarbituric acid reactive substances (TBARs), AFU, TNF-α, total glutathione, molecular docking, histological immunohistochemical markers anti-transforming growth factor-β1 (TGFβ1), double-strand DNA damage (H2AX), regeneration (KI67) apoptosis (Active caspase3) done. Results Discussion: Compared to toxin, treatment significantly ameliorated hepatoxicity as; hemoglobin, hematocrit hepatic glycogen, KI-67 immune-reactive increased. Although, inflammation, fibrosis, TGFβ1 immunoexpressing H2AX active caspase positive decreased. Noteworthy, curcumin's therapeutic effect was superior histomorphometry parameters. Furthermore, docking the structural influence on sequence showed higher binding affinity than taurine. Conclusion: Both induced strong antioxidative agents with more effectiveness curcumin.