Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometry

• DOI: 10.3389/fmolb.2023.1254699
• Publication Date: 2023-11-01T16:55:09Z
• Authors: Diana Buzova; Jan Frohlich; Danica Zapletalova; Marco Raffaele; Oriana Lo Re; Desislava K. Tsoneva; Jaroslav Sterba; Jan Cerveny; Manlio Vinciguerra
• Keywords: Ependymoma

Introduction: Pediatric brain tumours (PBT) are one of the most common malignancies during childhood, with variable severity according to location and histological type. Certain types gliomas, such a glioblastoma diffuse intrinsic pontine glioma (DIPG), have much higher mortality than ependymoma medulloblastoma. Early detection PBT is essential for diagnosis therapeutic interventions. Liquid biopsies been demonstrated using cerebrospinal fluid (CSF), mostly restricted cell free DNA, which display limitations quantity integrity. In this pilot study, we sought demonstrate detectability robustness histones in CSF. Methods: We collected CSF samples from cohort 8 children including DIPG, medulloblastoma, glioblastoma, others. As controls, nine unrelated blood without tumours. applied multichannel flow imaging approach on ImageStream(X) image indiviual histone or complexes different channels. Results: Single (H2A, macroH2A1.1, macroH2A1.2 H2B, H3, H4 H3 bearing H3K27M mutation), specifically detectable patients. H2A its variants macroH2A1.1/macroH2A1/2 displayed strongest signal abundance, together disease associated H3K27M. contrast, pediatric patients malignancies. Discussion: conclusion, strong affected by tumours, technology may provide additive diagnostic predictive information.