Jugular Vein Catheter Design and Cocaine Self-Administration Using Mice: A Comprehensive Method

• DOI: 10.3389/fnbeh.2022.880845
• Publication Date: 2022-06-15T16:02:11Z
• Authors: Gia Valles; Jessica L. Huebsc...; Elsbeth Chow; Corinne Kelly; Yuhong Guo; Laura N. Smith
• Keywords: Self-administration; Jugular vein; Drugs of abuse; Drug Administration

Intravenous self-administration (IVSA) is a behavioral method of voluntary drug intake in animal models which used to study the reinforcing effects drugs abuse. It considered have greater face validity substance use and abuse than other assays, thus, allows for valuable insight into neurobiological basis addiction, development disorders. The technique typically involves surgically inserting catheter jugular vein, enables infusion solution after performance desired operant behavior. Two nose- poke ports or levers are offered as manipulanda randomly assigned active (reinforced) inactive (non-reinforced) allow examination discrimination assessment learning. Here, we describe our methodological approach this assay mouse model, including construction surgical implantation vein catheter, set up chambers, considerations during each phase task.