MELK is a prognostic biomarker and correlated with immune infiltration in glioma

• DOI: 10.3389/fneur.2022.977180
• Publication Date: 2022-10-24T05:11:40Z
• Authors: Haiyan Yang; Huandi Zhou; Guohui Wang; Lei Tian; Haonan Li; Yufeng Zhang; Xiaoying Xue
• Keywords: 0301 basic medicine; 03 medical and health sciences; Neurology; immune infiltration; glioma; MELK; tumor microenvironment; prognosis; Neurology. Diseases of the nervous system; RC346-429; 3. Good health

Glioma accounts for the vast majority of primary brain tumors with inevitable recurrence and poor prognosis. Maternal embryonic leucine zipper kinase (MELK) is overexpressed in multiple human could activate a variety oncogenic-associated signal pathways. However, its role glioma microenvironment still largely unknown.We collected RNA sequence data clinical information gliomas from Chinese Genome Atlas (CGGA), The Cancer (TCGA), Gene Expression Omnibus (GEO) databases, investigated MELK expression correlation clinicopathologic features prognosis glioma. Moreover, relationship between immune cell infiltration tumor was explored through single-sample gene set enrichment analysis (ssGSEA) CIBERSORT. In addition, (GSEA) Metascape online were performed to find out signaling pathways enriched by differentially expressed genes (DEGs) high- low-MELK groups. Finally, immunohistochemistry validate our findings.Data CGGA GEO datasets showed that significantly upregulated than normal tissues, obviously correlated features, including age, WHO grade, histological subtype, IDH mutant status, 1p19q codeletion PRS type. Stratified analysis, Cox regression nomogram model revealed high predicted survival; hence, serve as an independent prognostic biomarker results pathway indicated system process, angiogenesis, apoptosis, cycle, other oncogenic-related Immune demonstrated increased higher scores, fractions immunocytes (T cells, NK cells resting, macrophages, resting mast neutrophils), levels checkpoints (B7-H3, CTLA4, LAG3, PD-1, PD-L1, TIM3). validated findings relates malignancy glioma.Our identified act indicator potential immunotherapy target conclusion, these suggested DDOST mediated immunosuppressive be important diagnosing treating gliomas.