Intra-Arterial Stem Cell Transplantation in Experimental Stroke in Rats: Real-Time MR Visualization of Transplanted Cells Starting With Their First Pass Through the Brain With Regard to the Therapeutic Action

• DOI: 10.3389/fnins.2021.641970
• Publication Date: 2021-03-02T05:39:24Z
• Authors: Daria D. Namestni...; Ilya L. Gubskiy; Veronica A. Revkova; Kirill K. Sukhinich; Pavel A. Melnikov; Anna N. Gabashvili; Elvira A. Cherkas...; Daniil A. Vishnev...; Victoria V. Kurilo; Veronica V. Burunova; Alevtina S. Semkina; Maxim A. Abakumov; Leonid V. Gubsky; Vladimir P. Chekh...; Jan-Eric Ahlfors; Vladimir P. Bakla...; Konstantin N. Yar...
• Keywords: Homing (biology); Stroke; Cell therapy

Cell therapy is an emerging approach to stroke treatment with a potential limit brain damage and enhance its restoration after the acute phase of disease. In this study we tested directly reprogrammed neural precursor cells (drNPC) derived from adult human bone marrow in rat middle cerebral artery occlusion (MCAO) model ischemic using placenta mesenchymal stem (pMSC) as positive control previously confirmed efficacy. Cells were infused into ipsilateral (right) internal carotid male Wistar rats 24 h MCAO. The main goal work was evaluate real-time distribution subsequent homing transplanted brain. This achieved by performing intra-arterial infusion inside MRI scanner allowed tracing starting their first pass through vessels. Immediately transplantation, observed periphery infarct zone stem, 15 min later small numbers could be discovered deep core contralateral hemisphere, where drNPC seen earlier greater than pMSC. Transplanted both groups no longer detected 48–72 infusion. Histological histochemical analysis demonstrated that pMSC localized blood vessels close contact vascular wall. No passage labeled barrier observed. Additionally, therapeutic effects compared. Both induced substantial attenuation neurological deficits evaluated at 7th 14th day transplantation modified severity score (mNSS). Some pMSC, such influence on volume survival rate animals, differed. results suggest paracrine mechanism IA infusion, potentially enhanced cell-cell interactions. Our data also indicate long-term not necessary for brain’s functional recovery.