Identification of the Potential Gene Regulatory Networks and Therapeutics in Aged Mice With Postoperative Neurocognitive Disorder

• DOI: 10.3389/fnins.2021.689188
• Publication Date: 2021-06-24T08:07:30Z
• Authors: Wensi Wu; Yongpai Peng; Jiaxin Zhou; Xiaojun Zhang; Lin Cao; Wei-jye Lin; Yanan Lu; Jing Wen; Zhi Wang
• Keywords: Neurocognitive; Competing Endogenous RNA

Postoperative neurocognitive disorder (PND) is one of the most common postoperative neurological complications in aged patients, characterized by mental disorder, anxiety, personality changes, and impaired memory. At present, molecular mechanism PND remains largely unclear, ideal biomarker for clinical diagnosis prognosis are lacking. Circular RNA (circRNA) microRNA (miRNA), as unique non-coding RNAs, affecting regulation miRNAs on genes further intervening progression diseases through sponge action between two. Besides, it could be served novel biomarkers various diseases. In order to detect differential expression profiles caused PND, a total 26 18-month-old male C57BL/6 mice were randomly assigned control group group. Behavioral tests showed that had cognitive function compared with Three each selected harvest brain analysis expressions circRNAs, miRNAs, mRNAs prefrontal cortex next-generation sequencing (NGS) technology. Differentially expressed genes, including 1192 27 266 identified, its accuracy was confirmed qRT-PCR. Bioinformatics results suggested neuroinflammation main pathological PND. The construction competitive endogenous (ceRNA) networks identification hub provided possible therapeutic targets Cinnarizine Clemastine predicted have potential effects This first study explore their mechanisms our new clues treatment this refractory disease.