Vascular responses of penetrating vessels during cortical spreading depolarization with ultrasound dynamic ultrafast Doppler imaging

• DOI: 10.3389/fnins.2022.1015843
• Publication Date: 2022-11-16T05:39:57Z
• Authors: Bao-Yu Hsieh; Yu-Chieh Jill Kao; Ning Zhou; Yi-Pei Lin; Yu-Ying Mei; Sung-Yu Chu; Dong-Chuan Wu
• Keywords: Cortical Spreading Depression; Arteriole; Haemodynamic response

The dynamic vascular responses during cortical spreading depolarization (CSD) are causally related to pathophysiological consequences in numerous neurovascular conditions, including ischemia, traumatic brain injury, cerebral hemorrhage, and migraine. Monitoring of the hemodynamic penetrating vessels CSD is motivated understand mechanism neurological disorders. Six SD rats were used, craniotomy surgery was performed before imaging. CSDs induced by topical KCl application. Ultrasound ultrafast Doppler used access changes, blood volume (CBV) flow velocity CSD, further analyzed those a single arteriole or venule. CSD-induced changes with typical duration propagation speed detected cortex ipsilateral induction site. hemodynamics typically showed triphasic initial hypoperfusion prominent hyperperfusion peak, followed long-period depression CBV. Moreover, different between individual arterioles venules proposed quantification CBV velocity. negative correlation basal change also reported vessels. These results indicate specific dynamics possibly contributions CSD-related pathological consequences. We using ultrasound imaging investigate responses. With this platform, it has potential monitor injuries advance.