Phenotypic and Functional Characterizations of Mesenchymal Stem/Stromal Cells Isolated From Human Cranial Bone Marrow

• DOI: 10.3389/fnins.2022.909256
• Publication Date: 2022-06-07T05:34:05Z
• Authors: Kaichuang Yang; Ruijie Lu; Jianan Lu; Shucai Fan; Qiang Zhang; Zijian Lou; Yuyuan Ma; Gang Lu; Ruolang Pan; Jianmin Zhang
• Keywords: CD90

Mesenchymal stem/stromal cells (MSCs) are adult stem that were originally isolated from bone marrow. In contrast to long bone-derived MSCs have been extensively characterized, our knowledge regarding flat bones (e.g., cranial bones) remain less clear. this study, purified human marrow (CB-MSCs) and their transdifferentiation capacity immunomodulatory functions further characterized. Phenotypic analysis of CB-MSCs demonstrated high expression CD73, CD90, CD105 while negative for CD14, CD34, HLA-DR. Further in vitro differentiation assay shown capable differentiating into cell types mesenchymal origin (i.e., adipocytes, osetoblasts, chondrocytes) collectively, these results indicated study bona fide according the minimal criteria proposed by International Society Cellular Therapy. Following expansion, single colony-derived (scCB-MSCs) obtained confocal microscopy revealed functional heterogeneity within primary CB-MSCs. Specifically, scCB-MSCs exhibited GABA progenitor features, as determined olig2 nestin. As expect, readily induced differentiate GABAergic neuron-like cells. Furthermore, roles evaluated following co-culture with peripheral blood lymphocytes co-culturing significantly suppressed lymphocyte proliferation promoted regulatory T but not Th1/Th17 phenotype. Overall, clonal marked propensity neural-like might represent promising candidates treatment neurodegenerative diseases.