Modulation of entorhinal cortex–hippocampus connectivity and recognition memory following electroacupuncture on 3×Tg-AD model: Evidence from multimodal MRI and electrophysiological recordings

• DOI: 10.3389/fnins.2022.968767
• Publication Date: 2022-07-29T05:58:56Z
• Authors: Bingbing Lin; Lanlan Zhang; Xiaolong Yin; Xiaocheng Chen; Chendong Ruan; Tiecheng Wu; Zhizhen Liu; Jia Huang
• Keywords: Subiculum; Entorhinal cortex; Infralimbic cortex

Memory loss and aberrant neuronal network activity are part of the earliest hallmarks Alzheimer’s disease (AD). Electroacupuncture (EA) has been recognized as a cognitive stimulation for its effects on memory disorder, but whether different brain regions or neural circuits contribute to recovery in AD remains unknown. Here, we found that deficit was ameliorated 3×Tg-AD mice with EA-treatment, shown by increased number exploring time spent novel object. In addition, reduced locomotor observed mice, no significant alteration seen EA-treated mice. Based functional magnetic resonance imaging, regional spontaneous alterations were mainly concentrated accumbens nucleus, auditory cortex, caudate putamen, entorhinal cortex (EC), hippocampus, insular subiculum, temporal visual so on. While EA-treatment prevented chaos parts above regions, such EC, cortex. And then used whole-cell voltage-clamp recording reveal neurotransmission reversed synaptic release. Since hippocampus receives most projections hippocampus-EC circuit is one related impairment. We further applied diffusion tensor imaging (DTI) tracking connectivity, hypo-connected between EC EA-treatment. These data indicate hippocampus–EC connectivity responsible recognition provide insight into potential therapies AD.