Omega-conotoxin MVIIA reduces neuropathic pain after spinal cord injury by inhibiting N-type voltage-dependent calcium channels on spinal dorsal horn

• DOI: 10.3389/fnins.2024.1366829
• Publication Date: 2024-02-26T04:31:50Z
• Authors: Nobuko Ohashi; Daisuke Uta; Masayuki Ohashi; Rintaro Hoshino; Hiroshi Baba
• Keywords: Nerve Injury; Allodynia

Spinal cord injury (SCI) leads to the development of neuropathic pain. Although a multitude pathological processes contribute SCI-induced pain, excessive intracellular calcium accumulation and voltage-gated calcium-channel upregulation play critical roles in However, role blockers pain is unknown. Omega-conotoxin MVIIA (MVIIA) blocker that selectively inhibits N-type voltage-dependent channels demonstrates neuroprotective effects. Therefore, we investigated spinal analgesic actions cellular mechanisms underlying effects SCI. We used model rats conducted behavioral tests, immunohistochemical analyses, electrophysiological experiments ( vitro whole-cell patch-clamp recording vivo extracellular recording). A behavior study suggested intrathecal administration acute phase after SCI induced analgesia for mechanical allodynia. Immunohistochemical recordings induces by directly inhibiting neuronal activity superficial dorsal horn. In showed presynaptic expressed on primary afferent Aδ-and C-fiber terminals suppresses glutamate release from substantia gelatinosa conclusion, may induce horn, resulting decreased excitability enhanced