Tryptophan metabolism and gut flora profile in different soybean protein induced enteritis of pearl gentian groupers

• DOI: 10.3389/fnut.2022.1014502
• Publication Date: 2022-12-19T14:15:09Z
• Authors: Wei Zhang; Aobo Pang; Beiping Tan; Yu Xin; Yu Liu; Ruitao Xie; Haitao Zhang; Qihui Yang; Junming Deng; Shuyan Chi
• Keywords: Flora

The substitution of high-level soy meals for fish meal (FM) generally leads to enteritis, accompanied by significant variations in gut flora. Relevant studies have pointed out a close relationship between tryptophan metabolism mediated flora and vertebrate inflammatory bowel disease. Present study examines the role profile enteritis caused different soybean meals. 960 groupers were randomly assigned into 4 groups (n = 4), which including: (1) FM (the control group, fed with 50% feed), (2) SBM40 (replacing 40% meal), (3) SPC40 protein concentrate), (4) FSBM40 fermented meal). Under average temperature natural light, cultivated feeds iso-nitrogen iso-lipid 10 weeks. results showed that at all experimental levels had negative effects on physiology growth performance. Typical features fluctuations immune system occur, can be observed enzyme activities total superoxide dismutase lysozyme contents immunoglobulin M, complement 3 4. 16SrDNA high-throughput sequencing indicated it greatly influenced abundance maleficent bacteria, like Vibrio, amplified increasing dietary According "3 + 2" full-length transcriptome sequencing, three inhibited key gene expressions metabolic pathway gut, however, there are some differences types genes inhibited. canonical correlation analysis changes positive pro-inflammatory (P < 0.05) negatively correlated expression anti-inflammatory 0.05). It is speculated from this closely related meal-related abnormal intestinal imbalance may play an important role. In future research, we further tolerance feed two aspects changes.