Integrated analysis of scRNA-seq and bulk RNA-seq reveals that GPRC5A is an important prognostic gene in pancreatic cancer and is associated with B-cell Infiltration in pancreatic cancer

• DOI: 10.3389/fonc.2024.1283164
• Publication Date: 2024-04-03T05:11:53Z
• Authors: Chunlu Dong; Haidong Ma; Ningning Mi; Wenkang Fu; Jianfeng Yi; Long Gao; Haiping Wang; Yanxian Ren; Yanyan Lin; Fangfang Han; Zhou Chen; Wence Zhou
• Keywords: Infiltration (HVAC)

Introduction Pancreatic cancer (PC) is a malignancy with poor prognosis. This investigation aimed to determine the relevant genes that affect prognosis of PC and investigate their relationship immune infiltration. Methods : First, we acquired single-cell chip data from GEO database scrutinize dissimilarities in cell infiltration differential between cancerous adjacent tissues. Subsequently, combined clinical TCGA identify Employing Cox Lasso regression analyses, constructed multifactorial prognostic model, which subsequently confirmed. The gene expression was authenticated using RT-PCR. Moreover, employed TIMER online examine T B Additionally, GPRC5A its correlation cells patient were ascertained tissue chips multiple fluorescence staining. Results analysis unveiled B-cell neighboring We developed model utilizing three genes, indicating patients high-risk scores experienced more unfavorable Immune revealed significant among YWHAZ, GPRC5A, In samples, exhibited substantial overexpression robust association an adverse prognosis, demonstrating positive Conclusion independent risk factor correlated PC. These outcomes indicated viable target for treating