Ampholytic ion-exchange magnetic beads: a promising tool for selecting short fragments in circulating cell-free DNA analysis

• DOI: 10.3389/fonc.2024.1397680
• Publication Date: 2024-05-08T04:27:05Z
• Authors: Gan He; Weixuan Wang; Yongxia Zhou; Guowei Zhao; Juan Liao
• Keywords: Liquid biopsy

Objective For liquid biopsy of cancer, the extraction circulating cell-free DNA (cfDNA) from plasma is required. We evaluated efficacy use magnetic submicron particles coated with abundant small zwitterions (MSP-ZEWBs) for extracting short fragments cfDNA. Methods developed and optimized an MSP-ZEWB-based cfDNA method using ampholytic ion-exchange materials compared its results those a control kit. measured concentration by quantitative polymerase-chain-reaction Qubit analyzed fragmentation patterns bioanalyzer. Results The fragment size isolated glycine hydrochloric acid at pH 2.2 exhibited better alignment marker. highest intensity was observed final 0.8% polyethylene glycol 8000. decreased significantly when marker MSP-ZEWBs adsorption buffer containing guanidine hydrochloride or isothiocyanoguanidine. All were successfully extracted both phosphate-buffered saline. Notably, remained consistent recovery long fragments. indicating potential selective Conclusion requires no protein denaturation, shows resistance to cells remaining in plasma, demonstrates higher overall efficiency reproducibility than other methods. Use may greatly enhance cancers through analysis clinical practice.