The role and mechanism of “eight famous herbals in Zhejiang” in cancer via network pharmacology and experimental validation

• DOI: 10.3389/fonc.2024.1475000
• Publication Date: 2024-11-15T05:10:23Z
• Authors: Ziheng Ni; Hao Zhang; Fengyun Chen; Mengjie Yang; Liting Yang; Yuan Zhou; Xianmin Zhou; Jiayi Guo; Xinyu Rao; Jiaqi Cen; Qun Lv; Jianjun Wang; Lailing Du; Gongxing Chen; Shuiping Liu
• Keywords: KEGG

In recent years, some components and active ingredients from the herbal formula “eight famous herbals in Zhejiang” (Zhe-Ba-Wei) have been reported to possess antitumor properties. However, there is still no systemic study on role mechanism of Zhe-Ba-Wei cancer. To systematically investigate anticancer efficacy Zhe-Ba-Wei, we first identified 17 with gene targets associated various types tumors. Second, screened these their responding multiple shared by analyzing convergence diverse tumor-specific target sites four crucial (ferulic acid, quercetin, rutin, luteolin), which were characterized 27 overlapping targets. Third, subsequently mapped onto Kyoto Encyclopedia Genes Genomes (KEGG) pathway Gene Ontology term, among total potential targets, 12 involved plasma membrane function. Fourth, investigated binding affinities between such as EGFR MET, both are well-known oncogenes cancers. Subsequently, an investigation computational ADMET properties showed that most exhibited good Finally, found three luteolin, quercetin) could inhibit proliferation non-small cell lung cancer cells decrease protein expression a concentration-dependent manner. All results shed light bioactive components, pharmacological effects, drug development utilization aiming provide useful support for its further research clinical application.