Effects of High-Pressure Processing, UV-C Irradiation and Thermoultrasonication on Donor Human Milk Safety and Quality

• DOI: 10.3389/fped.2022.828448
• Publication Date: 2022-03-26T11:56:17Z
• Authors: Eva Kontopodi; Bernd Stahl; Johannes B. van G...; Sjef Boeren; Rian A. H. Timmer...; Heidy M. W. den B...; Ruurd M. Van Elburg; Kasper Hettinga
• Keywords: Pasteurization; Lactoferrin; Pascalization

Holder pasteurization (HoP) is the current recommended treatment for donor human milk. Although this method inactivates microbial contaminants, it also negatively affects various milk components. High-pressure processing (HPP, 400, 500, and 600 MPa), ultraviolet-C irradiation (UV-C, 2,430, 3,645, 4,863 J/L) thermoultrasonication (TUS, 1,080 1,620 kJ/L) were investigated as alternatives to thermal (HoP). We assessed effects of these methods on microbiological safety, concentration functionality immunoglobulin A, lactoferrin, lysozyme bile salt-stimulated lipase, with LC-MS/MS-based proteomics activity assays. HoP, HPP, TUS, UV-C at 4863 J/L, achieved >5-log 10 reduction. Native protein levels showed highest reduction following while no significant was found after less intense HPP all treatments. Immunoglobulin contents preserved low intensity but lipase significantly reduced. This study demonstrated that may be considered suitable since they able ensure sufficient inactivation same time better preserving bioactive components In summary, our results provide valuable insights regarding evaluation selection treatment, which replace HoP in future.