Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells

• DOI: 10.3389/fphar.2022.1027315
• Publication Date: 2022-09-29T06:50:26Z
• Authors: Daniel Augustynowicz; Marta Kinga Lemie...; Jakub Władysław S...; Adrian Wiater; Michał Tomczyk
• Keywords: Ellagic Acid; Potentilla; Chlorogenic Acid; Quinic acid; Tannic acid; Astragalin; Tannin; Protocatechuic acid

Cinquefoils have been widely used in local folk medicine Europe and Asia to manage various gastrointestinal inflammations and/or infections, certain forms of cancer, thyroid gland disorders, wound healing. In the present paper, acetone extracts from aerial parts selected Potentilla species, namely P. alba (PAL7), argentea (PAR7), grandiflora (PGR7), norvegica (PN7), recta (PRE7) , closely related Drymocalis rupestris (syn. ) (PRU7), were analysed for their cytotoxicity antiproliferative activities against human colon adenocarcinoma cell line LS180 epithelial CCD841 CoN. Moreover, quantitative assessments total polyphenolic (TPC), tannin (TTC), proanthocyanidins (TPrC), flavonoid (TFC), phenolic acid (TPAC) conducted. The analysis secondary metabolite composition was carried out by LC-PDA-HRMS. highest TPC TTC found PAR7 (339.72 246.92 mg gallic equivalents (GAE)/g extract, respectively) PN7 (332.11 252.3 GAE/g respectively). TPrC, TFC, TPAC levels PAL7 (21.28 catechin (CAT)/g 71.85 rutin (RE)/g 124.18 caffeic (CAE)/g LC-PDA-HRMS revealed presence 83 compounds, including brevifolincarboxylic acid, ellagic pedunculagin, agrimoniin, chlorogenic astragalin, tiliroside. tri-coumaroyl spermidine demonstrated first time genus . Results MTT assay that all tested decreased viability both lines; however, a markedly stronger effect observed cancer cells. selectivity PAR7, which effectively inhibited metabolic activity cells (IC 50 = 38 μg/ml), while at same causing lowest unwanted effects CoN 1,134 μg/ml). BrdU significant decrease DNA synthesis examined lines response investigated extracts. It should be emphasized had on than normal cells, most properties case 174 μg/ml) 169 results LDH not cytotoxic whereas compounds significantly damaged membranes, dose-dependent. which, concentrations ranging 25 250 μg/ml, increased release 110%–1,062%, respectively. Performed studies species may useful sources anti-colorectal agents; additional research is required prove this definitively.