Integrative proteomics and metabolomics approach to identify the key roles of icariin-mediated protective effects against cyclophosphamide-induced spermatogenesis dysfunction in mice

• DOI: 10.3389/fphar.2022.1040544
• Publication Date: 2022-12-14T06:16:31Z
• Authors: Chunyan Fang; Yulong Ye; Fang Yang; Fangyue Wang; Yifeng Shen; Degui Chang; Yaodong You
• Keywords: Icariin

The alkylating antineoplastic agent cyclophosphamide (CP) is known to be toxic the male reproductive system, but there are no effective prevention or treatment options. flavonoid icariin (ICA), which used in Chinese medicine, has been shown have a number of biological functions, including testicular protection. current study looked into protective effects ICA preventing CP-induced spermatogenesis dysfunction. role dysfunction caused by CP. For 5 days, healthy adult mice were given saline single dose CP (50 mg/kg) intraperitoneally (i.p). next 30 (80 gavage. Animals euthanized 12 h after receiving ICA, and testes removed for biochemical, histopathological, sperm evaluation, transmission electron microscope analysis (TEM). We also investigated potential on using an integrated proteomic metabolomic approach. levels 8309 proteins 600 metabolites measured. majority differential found enriched variety metabolic pathways, PI3K-Akt signaling pathway, necroptosis, mTOR glycerophospholipid metabolism, ABC transporters, implying that may molecular mechanisms contribute testis. Taken together, these findings show effectively reduces testis injury, infertility preservation.