Rutaecarpine Attenuates Oxidative Stress-Induced Traumatic Brain Injury and Reduces Secondary Injury via the PGK1/KEAP1/NRF2 Signaling Pathway

• DOI: 10.3389/fphar.2022.807125
• Publication Date: 2022-04-12T07:24:02Z
• Authors: Min Xu; Liu Li; Hua Liu; Wei Lu; Xiaoyang Ling; Mingjie Gong
• Keywords: KEAP1; Rutaecarpine

The oxidative stress response caused by traumatic brain injury (TBI) leads to secondary damage in the form of tissue and cell death. Nuclear transcription-related factor 2 (NRF2) is a key body against has an important role combating TBI neurons. In present study, we investigated whether rutaecarpine could activate PGK1/KEAP1/NRF2 pathway antagonize We performed controlled cortical impact (CCI) surgery on mice taken H O treatment PC12 cells construct models. results western blot showed that expression PGK1, KEAP NRF2 was regulated accompanied altered levels stress, use model significantly improved cognitive dysfunction, increased antioxidant capacity reduced apoptosis tissue. Similar were obtained using model. Furthermore, through protein synthesis inhibitor CHX proteasome MG-132, found promote expreesions PGK1 accelerating ubiquitination reduce expression. Therefore, may be promising therapeutic agent for TBI-related neuro-oxidative damage.