Hydrogen Sulfide Promotes Thyroid Hormone Synthesis and Secretion by Upregulating Sirtuin-1

• DOI: 10.3389/fphar.2022.838248
• Publication Date: 2022-02-10T08:54:24Z
• Authors: Xue Zhao; Yedi Cao; Hongfang Jin; Xiuli Wang; Lanbo Zhang; Yang Zhang; Yang Yu; Youyuan Huang; Ying Gao; Junqing Zhang
• Keywords: Sirtuin 1

Objective: One mechanism of hypothyroidism involves the disruption thyroid hormone synthesis and secretion by thyrocytes. Hydrogen sulfide (H2S), as a gas signaling molecule, participates in many physiopathologic processes upregulating sirtuin-1 (SIRT1). The aim current study was to explore whether H2S promotes hormones SIRT1. Methods: Real-time PCR immunohistochemistry were used detect mRNA protein expression H2S-generating enzymes normal human tissues. Serum concentrations from hypothyroid patients (n = 32) euthyroid participants 41) detected H2S-selective sensors. Thirty-one Sprague-Dawley rats divided into control group 10), (induced MMI, n 10) + NaHS 11), FT4, TT4 TSH levels assayed. Human primary thyrocytes incubated with donor sodium hydrosulfide (NaHS) or plus SIRT1 inhibitor (EX527) vitro. Thyroid synthesis- secretion-related proteins [thyroid peroxidase (TPO), iodide transporter (NIS), Pendrin, monocarboxylic acid 8 (MCT8)] analyzed real-time Western blot. Results: serum decreased compared those (p < .05), positively correlated FT3, TT3, all subjects (all p .0001). In vivo, promoted function .05). vitro, supernatant, CBS higher than CSE 3-MPST TPO, NIS, Pendrin MCT8 upregulated concentration-dependent manner for After blocking EX527, we found that increasing TPO activity downregulated NaHS, FT4 cell supernatant also significantly Conclusion: is mainly generated CBS. are hypothyroidism. related molecules